Table 3.
Phenolic compounds identified in C. chinense leaves by UPLC-PDA-ESI-MS/MS in the extract obtained using 50% MeOH by UAE and hydrolyzed extract.
| Number | TR | PDA UV bands (nm) | Experimental Accurate Mass |
Molecular Formula | Fragments (m/z) |
Tentative identification | ||
|---|---|---|---|---|---|---|---|---|
| [M–H] - | [M + H] + | Ion negative | Ion positive | |||||
| 1 | 7.941 | 194, 213, 293, 317 | 249.0850 | 251.1308 | C13H18N2O3 | 249 (1 0 0) | 251 (95) 234 (21) 163 (1 0 0) 72 (80) | N-caffeoyl putrescine (Isomer I) |
| 2 | 8.130 | 194, 213, 293, 317 | 249.0850 | 251.1308 | C13H18N2O3 | 249 (1 0 0) | 251 (92) 234 (21) 163 (1 0 0) 72 (61) | N-caffeoyl putrescine (Isomer II) |
| 3 | 8.550 | 197, 218, 278 | 315.0823 | 227.1421 | C13H16O9 | 315 (1 0 0) 203 (54) | 227 (18) 188 (1 0 0) 146 (91) 118 (28) 100 (17) | Protocatechuic acidhexoside |
| 4 | 8.740 | 212, 299, 325 | 353.0936 | C16H18O9 | 353 (1 0 0)215 (13) 293 (19) 191 (82) | 5-Caffeolquinic acid | ||
| 5 | 9.080 | 209, 284 | 215.0827 | 217.1303 | C12H12N2O2 | 215 (1 0 0) | 217 (47) 200 (95) 88 (1 0 0) | Unknown |
| 6 | 9.235 | 205, 270, 297, 338 | 593.1991 | 595.2100 | C27H30O15 | 593 (72) 431 (9) 302 (9) 177 (1 0 0) | 595 (7) 471 (1 0 0) 144 (57) 100 (31) | Unknown |
| 7 | 9.540 | 195, 213, 312 | 329.0902 | C14H18O9 | 329 (55) 265 (62) 177 (1 0 0) | Vanillic Acid-4-β-D-glucoside | ||
| 8 | 9.860 | 272, 320 | 597.2781 | C27H34O15 | 387 (1 0 0) 597 (9) | Unknown | ||
| 9 | 10.485 | 210, 306 | 337.1141 | C16H18O8 | 337 (1 0 0) 191 (89) | Coumaroylquinic acid | ||
| 10 | 10.904 | 205, 254, 348 | 579.1603 | 581.1707 | C26H28O15 | 579 (1 0 0) | 581 (1 0 0) 287 (18) | Luteolin-7-O-(2-O-apiosyl) hexoside |
| 11 | 12.730 | 199, 266, 336 | 563.1839 | 565.1846 | C26H28O14 | 563 (1 0 0) | 565 (1 0 0) 433 (9) 271 (29) | Apigenin-7-O-(2-O-apiosyl) hexoside |
| 12 | 13.212 | 206, 251, 266, 347 | 593.1651 | 595.2073 | C27H30O15 | 593 (1 0 0) | 595 (1 0 0) 387 (25) 301 (14) | Diosmetin-7-O-(2-O-apiosyl) hexoside |
| 13 | 13.466 | 206, 253, 348 | 665.1592 | 667.1755 | C26H34O20 | 621 (1 0 0) 665 (57) | 667 (1 0 0) | Luteolin-7-O-(2-apiosyl-6-malonyl) hexoside |
| 14 | 17.761 | 208, 266, 336 | 649.1833 | 651.1900 | C29H30O17 | 649 (9) 635 (32) 605 (1 0 0) | 651 (1 0 0) | Apigenin-7-O-(6-malonyl-apiosyl) hexoside |
| 15 | 18.603 | 210, 251, 266, 347 | 679.1975 | 681.1900 | C23H40O20 | 679 (12) 635 (1 0 0) | 681 (1 0 0) | Chysoeriol-7-O-(6 malonyl-apiosyl) hexoside |
| 1* | 15.148 | 207, 253, 347 | 447.1658 | 449.1168 | C21H20O11 | 447 (1 0 0) 285 (90) 284 (80) | 449 (1 0 0) 287 (61) | Luteolin-7-O-hexoside |
| 2* | 20.271 | 206, 266, 336 | 431.1199 | 433.1222 | C21H20O10 | 431 (88) 268 (1 0 0) | 433 (1 0 0) 271 (79) | Apigenin-7-O-hexoside |
| 3* | 24.880 | 205, 251, 265, 347 | 461.1377 | 463.1222 | C22H22O11 | 461 (1 0 0) 297 (6) 283 (32) 255 (22) | 463 (1 0 0) 453 (30) 301 (44) | Diosmetin-7-O-hexoside |
| 4* | 26.505 | 210, 252, 267, 347 | 285.061 | 287.0468 | C15H10O6 | 285 (68) 133 (1 0 0) | 287 (1 0 0) | Luteolin |
| 5* | 27.359 | 214, 267, 336 | 269.0797 | 271.0555 | C15H10O5 | 269 (27) 117 (1 0 0) | 271 (1 0 0) 246 (34) | Apigenin |
| 6* | 27.529 | 210, 247, 267, 346 | 299.0699 | 301.0551 | C16H12O6 | 299 (1 0 0) 284 (49) 227 (46) 151 (30) 107 (35) | 301 (1 0 0) | Chrysoeriol |
*
Identified in the hydrolyzed extract.