Fig. 5.

Activation of apoptosis and other molecules after SH and iodine-131. HTori-3 BCPAP and TPC-1 cells were treated with or with 0.8 mM SH along, RAI along or SH plus RAI 14.8 MBq/ml. (A)The protein expression levels of p53, p21, Fas, Bcl-2, and Bax were determined by western blot analysis, GAPDH was probed as a loading control. The mRNA expression levels of (B)ratio of Bcl-2 to Bax, (C)Fas, (D) p53, and (E) p21 were determined by reverse transcription-quantitative polymerase chain reaction. The data are presented as the mean ± standard deviation of four independent experiments(*P<0.05). SH, sinomenine hydrochloride; I, iodine-131; ISH, co-treatment of iodine-131 and SH.