Fig. 6.

SH enhances expression of p-ATM and the iodine-131-induced activation of Chk1 and Chk2. SH and iodine-131 regulate the expression of p-Chk1(T68) p-Chk2(S345) and p-ATM in (A)HTori-3, (B)BCPAP and (C)TPC-1 cells. The protein expression levels of p-Chk1(T68) p-Chk2(S345) and p-ATM were determined by western blot analysis, Gapdh was probed as a loading control. The mRNA expression levels of (D) ATM mRNA were determined by reverse transcription-quantitative polymerase chain reaction. And normalized using GAPDH as an internal control (*P < 0.05). SH, sinomenine hydrochloride; I, iodine-131; ISH, co-treatment of iodine-131 and SH.