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. 2021 Jun 28;8:685330. doi: 10.3389/fnut.2021.685330

Figure 2.

Figure 2

Comparative residual activity (%) of soluble antiacanthain (0.075 mg/ml, 1.875 IU/ml) in 100 mM Tris(hydroxymethyl)amino methane hydrochloride buffer pH 8 vs. soluble antiacanthain (0.075 mg/ml) in almost anhydrous organic solvents (Xw: 1 × 10−5) and glyxoyl-silica immobilized antiacanthain (12.5 mg/ml) in almost anhydrous ethyl ethanoate (Xw: 1 × 10−5), after 6 h of incubation at 40°C under agitation at 200 rpm. (1) Control (Tris buffer pH 8, initial specific activity: 100%); (2) Tris buffer pH 8; (3) Diethyl ether; (4) Chlorobenzene; (5) Acetophenone; (6) Octanol; (7) Benzene; (8) Dichloromethane; (9) Cyclohexane; (10) Ethyl ethanoate (soluble antiacanthain); (11) Ethyl ethanoate (glyoxyl-silica immobilized antiacanthain).