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. 2021 Jun 28;8:685330. doi: 10.3389/fnut.2021.685330

Figure 3.

Figure 3

Component separation by RP-HPLC of a representative sample from Z-Tyr-Val-OH enzymatic synthesis under kinetic control, using: (A) antiacanthain (0.075 mg/ml, 1.875 IU/ml) and (B) granulosain (0.125 mg/ml, 1.875 IU/ml) as soluble biocatalysts, in 50% v/v ethyl ethanoate and 100 mM Tris(hydroxymethyl)amino methane hydrochloride buffer pH 8, after 4 h and 30 min of reaction, respectively; at 40°C under agitation at 200 rpm. I: antiacanthain (tR: 3–4.4 min) and granulosain (tR: 2,7–3,2 min); II: Z-Tyr-Val-OH (tR: 5.27 min); III: Z-Tyr-pNO (tR: 14.5 min). Aqueous phase: blue line. Organic phase: pink line.