. 2021 Jun 28;8:685330. doi: 10.3389/fnut.2021.685330

Table 2.

Catalytic potential of soluble granulosain (0.125 mg/ml, 1.850 IU/ml) after 6 h in liquid-liquid biphasic systems constituted by several concentrations of organic solvent in 100 mM Tris(hydroxymethyl)amino methane hydrochloride buffer pH 8, at 40°C.

Organic solvent	Catalytic potential (IU/mg · h)^*
	30%	50%	70%
Benzene	76.38 ± 0.15^a	139.42 ± 0.00^d	56.09 ± 0.24^d
Octanol	13.05 ± 0.09^e	17.92 ± 0.01^a	0 ± 0.00^a
Dichloromethane	121.57 ± 0.01^h	191.09 ± 0.01^g	29.98 ± 0.33^c
Diethyl ether	38.53 ± 0.00^c	25.47 ± 0.05^b	0 ± 0.00^a
Cyclohexane	92.31 ± 0.28^f	176.92 ± 0.03^e	57.90 ± 0.25^e
Chlorobenzene	73.18 ± 0.01^d	188.01 ± 0.01^f	61.15 ± 0.01^f
Acetophenone	16.45 ± 0.05^b	64.77 ± 0.20^c	0 ± 0.00^a
Ethyl ethanoate	117.56 ± 0.01^g	200.14 ± 0.01^h	4.42 ± 0.00^b

The catalytic potential of granulosain in 100 mM Tris(hydroxymethyl)amino methane hydrochloride buffer pH 8, at 40°C was 350 ± 0.01 (IU/mg · h).

Catalytic potential data are expressed as the mean ± SD (standard deviation) of three independent experiments which were done in duplicate. The values with different superscript letters in a column are significantly different (p < 0.05) (IBM SPSS Statistics 27.0).