Table 4.
Yields (η) obtained in the kinetically controlled synthesis of Z-Tyr-Val-OH, using 50% v/v ethyl ethanoate in 100 mM Tris(hydroxymethyl)amino methane hydrochloride buffer pH 8 as medium, and antiacanthain (0.075 mg/ml, 1.875 IU/ml) and granulosain (0.125 mg/ml, 1.850 IU/ml) as soluble biocatalysts, at 40°C under agitation at 200 rpm.
| Time (min) | Z-Tyr-Val-OH (mM) | αs (%) | η (%) | |||
|---|---|---|---|---|---|---|
| Antiacanthain Granulosain | Antiacanthain Granulosain | Antiacanthain Granulosain | ||||
| 0 | 0b | 0b | 0a | 0a | 0a | 0a |
| 1 | 0.35 ± 0.03c | 4.16 ± 0.05e | 4.6 ± 0.09b | 31.4 ± 0.05b | 2.45 ± 0.09b | 29.7 ± 0.03f |
| 5 | 1.75 ± 0.20d | 5.22 ± 0.10f | 23.2 ± 0.33c | 51.4 ± 0.10c | 10.1 ± 0.10c | 37.3 ± 0.07g |
| 15 | 2.20 ± 0.01e | 6.80 ± 0.05g | 29.4 ± 0.19d | 60.0 ± 0.25d | 12.75 ± 0.05d | 48.6 ± 0.03h |
| 30 | 4.30 ± 0.01f | 7.30 ± 0.10h | 41.3 ± 0.12e | 67.6 ± 0.09e | 24.93 ± 0.12e | 52.1 ± 0.10i |
| 60 | 7.53 ± 0.10g | 3.50 ± 0.09d | 63.1 ± 0.20f | 80.6 ± 0.45f | 43.65 ± 0.09f | 25.0 ± 0.15e |
| 150 | 8.53 ± 0.05h | 3.00 ± 0.10c | 76.9 ± 0.07g | 84.6 ± 0.52g | 49.45 ± 0.05g | 21.4 ± 0.09d |
| 240 | 9.22 ± 0.01a | 2.35 ± 0.01a | 91.3 ± 0.05h | 88.1 ± 0.09h | 53.45 ± 0.01h | 16.8 ± 0.05c |
| 360 | 9.02 ± 0.12a | 2.10 ± 0.20a | 100 ± 0.01i | 100 ± 0.01i | 52.28 ± 0.05i | 15.0 ± 0.10b |
Acyl acceptor/donor molar ratio: 1. αs is the conversion percentage of acyl donor substrate.
The values with different superscript letters in a column are significantly different (p < 0.05) (IBM SPSS Statistics 27.0).
Bold values represent the maximal peptide yields from each experimental trial set.