Table 5.
Yields (η) obtained in the kinetically controlled synthesis of Z-Tyr-Val-OH, using 50% v/v ethyl ethanoate in 100 mM Tris(hydroxymethyl)amino methane hydrochloride buffer pH 8 as medium, and antiacanthain (0.075 mg/ml, 1.875 IU/ml) and granulosain (0.125 mg/ml, 1.850 IU/ml) as soluble biocatalysts, at 40°C under agitation at 200 rpm.
| Time (min) | Z-Tyr-Val-OH (mm) | αs (%) | η (%) | |||
|---|---|---|---|---|---|---|
| Antiacanthain Granulosain | Antiacanthain Granulosain | Antiacanthain Granulosain | ||||
| 0 | 0b | 0a | 0a | 0b | 0a | 0a |
| 1 | 0.57 ± 0.32c | 2.45 ± 0.02b | 3.77 ± 0.10b | 14.2 ± 0.05c | 3.29 ± 0.0b | 14.2 ± 0.00b |
| 5 | 1.83 ± 0.01d | 6.16 ± 0.23f | 10.6 ± 0.32c | 35.7 ± 0.01d | 10.6 ± 0.05c | 35.7 ± 0.01f |
| 15 | 4.23 ± 0.05e | 8.16 ± 0.03h | 24.5 ± 0.01d | 52.7 ± 0.35e | 24.5 ± 0.10d | 47.3 ± 0.05h |
| 30 | 6.21 ± 0.05f | 10.3 ± 0.05i | 36.0 ± 0.14e | 76.3 ± 0.05f | 36.0 ± 0.02e | 60.0 ± 0.05i |
| 60 | 10.2 ± 0.01g | 7.33 ± 0.05g | 73.3 ± 0.05f | 81.2 ± 0.51g | 59.3 ± 0.01f | 42.5 ± 0.03g |
| 150 | 11.9 ± 0.07h | 5.71 ± 0.21e | 89.6 ± 0.45g | 93.5 ± 0.03h | 68.7 ± 0.05g | 33.1 ± 0.20e |
| 240 | 12.4 ± 0.01a | 4.23 ± 0.05d | 98.0 ± 0.05h | 100 ± 0.00a | 72.0 ± 0.01h | 24.5 ± 0.05d |
| 360 | 12.2 ± 0.25a | 3.67 ± 0.05c | 100 ± 0.00i | 100 ± 0.00a | 70.8 ± 0.05i | 21.0 ± 0.20c |
Acyl acceptor/donor molar ratio: 10. αs is the conversion percentage of acyl donor substrate.
The values with different superscript letters in a column are significantly different (p < 0.05) (IBM SPSS Statistics 27.0).
Bold values represent the maximal peptide yields from each experimental trial set.