Table 6.
Yields (η) obtained in the kinetically controlled synthesis of Z-Tyr-Val-OH in ethyl ethanoate (Xw: 1 × 10−5 in the enzyme into the organic solvent) at 40°C, using glyoxyl-silica immobilized antiacanthain (12.5 mg/ml) as biocatalyst.
| Time (min) | Z- Tyr-pNO (mM) | Z-Tyr-Val-OH (mM) | αs (%) | η (%) |
|---|---|---|---|---|
| 0 | 17.25 | 0 | 0a | 0a |
| 5 | 14.83 | 2.41 | 14 ± 0.20b | 14 ± 0.00b |
| 15 | 12.94 | 4.30 | 25 ± 0.37c | 25 ± 0.01c |
| 30 | 10.35 | 6.90 | 40 ± 0.00d | 40 ± 0.15d |
| 60 | 9.15 | 8.20 | 47 ± 0.10e | 47± 0.03e |
| 240 | 4.80 | 12.10 | 72 ± 0.00f | 70 ± 0.02f |
| 360 | 4.25 | 12.94 | 75 ± 0.18g | 75 ± 0.13g |
αs is the conversion percentage of acyl donor substrate.
The values with different superscript letters in a column are significantly different (p < 0.05) (IBM SPSS Statistics 27.0).
Bold values represent the maximal peptide yields from each experimental trial set.