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. 2021 Jun 28;8:676235. doi: 10.3389/fmolb.2021.676235

FIGURE 1.

FIGURE 1

The C-terminus of the UIM3 isoform of ataxin-3 is predicted disordered and includes a UIM. (A) The figure illustrates the amino acid sequence of AT-3306-361. The blue box indicates UIM3 of AT-3 (residues E336-T350). (B) The panel shows the residue-wise prediction of the S2 order parameter in AT-3306-361 from DynaMine (purple). The dashed lines show the thresholds of S2 predicted scores to classify residues as ordered (S2 ≥  0.75), context-dependent (0.65 < S2  < 0.75), and disordered (S2   ≤ 0.65). (C) The panel shows the residue-wise prediction of disorder propensity in AT-3306-361 from the Multilayered Fusion-based Disorder predictor v. 2.00 (MFDp2, green), and SPOT-Disorder2 (SPOTdis.2, yellow) predictors. The dashed line shows the threshold of disorder probability to classify the residues as disordered ( ≥ 0.5) and ordered ( < 0.5). (D) The plot shows the residue-wise prediction of secondary structure propensity (Coil orange, Helix violet, Strand teal) from PSI-PRED. The dashed line shows the threshold of secondary structure probability for each structural class to classify the residues as coil, helix or strand ( ≥ 0.5). The light blue box indicates UIM3. The predictors report AT-3306-361 as a mainly disordered tract with propensity to order and helical structures in proximity of UIM3.