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cDNA PCR-amplified regions of Asp n gene. (A) Full cDNA gene amplicon using primers Aspn3_FW and Aspn3_RV. (B) Conserved cDNA gene region amplified using primers CRPEROX_FW and CRPEROX_FW. Asp. Nig, Aspergillus niger (positive control). Lines 1–14: samples of mangoes inoculated with spores of A. niger. B1 and B2: samples of mangoes inoculated with phosphate-buffered saline. Asp. fum, Aspergillus fumigatus. C- :PCR negative control.
