Table 1.

Stock and working concentrations of reagents

Reagent	Solvent	Stock concentration	Working concentration	Storage conditions	Aliquot
Crotonate, pH 7.4	dH2O	1 M	5 mM	18°C–25°C for 1 year
Y-27632	dH2O	10 mM	10 μM	−20°C for 1 month	0.1 mL/tube
0.5 mM EDTA	DPBS		0.5 mM	18°C–25°C for 6 months	100 mL/bottle
CHIR99021	DMSO	5 mM	2 μM	−80°C for 2 years	0.1 mL/tube
Activin A	0.1% dFBS in DPBS	100 ng/mL	25 ng/mL	−80°C for 3 months	20 μL/tube