Table 2.

RACC endoderm differentiation medium

Reagent	Stock concentration	Working concentration	Volume
RPMI 1640	N/A	N/A	49.1 mL
0.2% dFBS	100%	0.2%	100 μL
Activin A	100 ng/mL	25 ng/mL	12.5 μL
CHIR99021	5 mM	2 μM	20 μL
MEM NEAA	100 ×	1 ×	0.5 mL
Crotonate, PH 7.4	1 M	5 mM	0.25 mL
Total	n/a	n/a	50 mL