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Cell number and medium volume in different plates
| Plate | Area (cm2) | Matrigel (mL) | Mel1 cells (1.05 × 104/cm2 | H9 cells (2.1 × 104/cm2) | STEMDIFF endoderm differentiation medium plus 5 mM crotonate (0.13–0.26 mL/cm2) | RACC endoderm differentiation medium (0.19 mL/cm2) |
|---|---|---|---|---|---|---|
| 24 well | 1.9 | 0.35–0.5 | 2.0 × 104 | 4.0 × 104 | 0.3–0.5 mL | 0.36 mL |
| 12 well | 3.8 | 0.5–1 | 4.0 × 104 | 8.0 × 104 | 0.5–1 mL | 0.73 mL |
| 6 well | 9.5 | 1.5–2 | 10.0 × 104 | 20.0 × 104 | 1.3–2 mL | 1.8 mL |
