Stock concentration	Final concentration	Amount
CG medium
MEM	N/A	1×	500 mL
FCS	N/A	10% (v/v)	50 mL
Sodium Pyruvate	100×	1 mM (1×)	5 mL
Penicillin / Streptomycin	100×	100 units/mL penicillin and 100 μg/mL streptomycin (1×)	5 mL
NEAA	100×	1×	5 mL

The CG medium can be store for 4 weeks at 4°C.