Stock concentration	Final concentration	Amount
TCS medium
DMEM w/o phenol red	N/A	1×	500 mL
CD-FCS	N/A	10% (v/v)	50 mL
Penicillin/Streptomycin	100×	100 units/mL penicillin and 100 μg/mL streptomycin (1×)	5 mL
Glutamine	100×	2 mM (1×)	5 mL

The TCS medium can be store for 4 weeks at 4°C.