Stock concentration	Final concentration	Amount
CT medium
DMEM w/o phenol red	N/A	1×	500 mL
CD-FCS	N/A	1% (v/v)	5 mL
Penicillin/Streptomycin	100×	100 units/mL penicillin and 100 μg/mL streptomycin (1×)	5 mL
Glutamine	100×	2 mM (1×)	5 mL

The CT medium can be store for 4 weeks at 4°C.