REAGENT or RESOURCE	SOURCE	IDENTIFIER
Critical commercial assays
Dual-Glo® Luciferase Assay System	Promega	Cat# E2940
Great EscAPe™ SEAP Chemiluminescence Kit 2.0	Takara	Cat# 631738
JetPEI®	Polyplus-transfection	Cat# 101-10N
Chemicals, peptides, and recombinant proteins
Rifampicin	Sigma-Aldrich	Cat# R3501
PK11195	Sigma-Aldrich	Cat# C0424
CITCO (6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde-O-(3,4-dichlorobenzyl)oxime)	Sigma-Aldrich	Cat# C6240
Phenobarbital (Gardenal)	Sanofi-Aventis	Cat# 3286209
Gibco™ EGF Recombinant Human Protein	Fischer Scientific	Cat# PHG0315
BD Matrigel Matrix Phenol Red-Free	BD Biosciences	Cat# 356237
QuantiLum® Recombinant Luciferase	Promega	Cat# E170A
Luciferase Inhibitor II	Calbiochem	Cat# 119114
ATP (10 mM)	Cell Signaling Technology	Cat# 9804
Beetle Luciferin, Potassium Salt	Promega	Cat# E1602
Polyoxyethylene-20 (TWEEN 20)	Bio Basic Canada Inc.	Cat# 9005-64-5
Albumin from bovine serum (BSA)	Sigma-Aldrich	Cat# A7030
Dextran, from Leuconostoc spp.	Sigma-Aldrich	Cat# 31390
Activated charcoal	Sigma-Aldrich	Cat# C9157
150 mM NaCl solution sterile filtred 0.2 μm	Polyplus-transfection	Cat# 702-50
PBS	Thermo Fischer Scientific	Cat#14190-094
Minimum Essential Medium (MEM )	Thermo Fischer Scientific	Cat# 31095-029
DMEM low glucose, pyruvate, no glutamine, no phenol red	Thermo Fischer Scientific	Cat# 11880036
MEM Non-Essential Amino Acids Solution (NEAA) (100×)	Thermo Fischer Scientific	Cat# 11140050
Penicillin/streptomycin	Thermo Fischer Scientific	Cat# 15140-122
Sodium Pyruvate 100 mM (100×)	Thermo Fischer Scientific	Cat# 11360-039
Glutamine 200 mM (100×)	Thermo Fischer Scientific	Cat# 25030-024
Trypsin-EDTA	Thermo Fischer Scientific	Cat# 25300-054
Fetal Calf Serum (FCS)	Eurobio	Cat# CVFSVF00-01
Fetal Bovine Serum, charcoal-stripped, USDA-approved regions	Thermo Fischer Scientific	Cat# 12676029
Paraformaldehyde	Sigma-Aldrich	Cat# P6148
Hoechst-33258 pentahydrate	Thermo Fischer Scientific	Cat# H3569
Dako Fluorescent Mounting Medium	Dako North America	Cat# S3023
Experimental models: cell lines
Human HepG2 cell line	ATCC	Cat# HB-8065
Recombinant DNAs
p(UAS)4-tk-Luc	Lien et al. 2014	N/A
pSG5-GAL4(DBD)-hPXR(LBD)	Lemaire et al. 2006	N/A
pM (Gal4 empty)	Clontech	Cat# PT3119-5
pGAL4-hCAR1	Hering et al. 2018	N/A
p(UAS)4-tk-SEAP	This article	N/A
p-RL (CMV) [renilla luciferase normalization vector]	Promega	Cat# E2261
p-EGFP-hCAR1	GeneCopoeia	Cat# EX-A0964-M29
pGal4-Rev-erbα	Hering et al. 2018	N/A
pVP16-NCoR	Hering et al. 2018	N/A
Other
Victor Light luminometer	PerkinElmer	Model 1420 Luminometer counter
Epifluorescence microscope	Leica Microsystems	Model DMI 6000B
Combitips advanced 5 mL	Eppendorf	Cat# 0030089669
Thermo Scientific Menzel X1000 Round Coverslip dia. 12 mm #1.5 (0.16–0.19mm)	Fischer Scientific	Cat# 11846933
6-cm Diameter plates	Falcon	Cat# 353004
6-Well plates	Thermo Fischer Scientific	Cat# 130184
24-Well plates	Thermo Fischer Scientific	Cat# 930186
White 96-well plates	Greiner Bio-One	Cat# 675074