Bacterial and virus strains |
|
Lentivirus carrying EF1-CBR2opt-T2A-copGFP |
Addgene |
Plasmid #108713 |
Lentivirus carrying EF1-CBG2-T2A-copGFP |
Mezzanotte Lab |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
NH2-NpLH2 substrate |
Promega/synthesis reported in (Hall et al., 2018) |
N/A |
Isoflurane Isoflutek 1000 mg/g |
Laboratorios Karizoo |
710004 |
Dulbecco's Phosphate Buffered Saline (1×), (DPBS) |
Lonza Bioscience |
BE17-513F |
Dulbecco’s modified Eagle’s medium (DMEM) |
Gibco |
11880028 |
|
Experimental models: Cell lines |
|
293T (HEK) cell line |
ATCC |
ATCC® CRL-3216™ |
|
Experimental models: Organisms/strains |
|
Nude mouse: BALB/C wild type |
Charles River Laboratories (The Netherlands) |
CAnN.Cg-Foxn1nu/Crl |
|
Software and algorithms |
|
Living Image software 4.5 and above |
PerkinElmer |
Part #128113 |
GraphPad Prism 7 software |
GraphPad |
N/A |
|
Other |
|
IVIS Spectrum Imager |
PerkinElmer |
124262 |
BC Insuline 1 mL U-100 29G, 0.33mm × 12.7mm 10× 10st, syringe, single use |
BD Biosciences |
Cat# 309623 |
Corning® 96 Well-Black Polystyrene Microplate |
Greiner Bio-one |
CLS3601-100EA |
T75 EasYFlask, TC Surface, Solid Cap, Pack of 5 |
Thermo Fisher Scientific |
156472 |