REAGENTS or RESOURCES	SOURCE	IDENTIFIER
Antibodies
Anti-phospho-AMPKa used at 1:1000	Cell Signaling Technology	Cat # 2535; RRID: AB_331250
Anti- AMPKa used at 1:1000	Cell Signaling Technology	Cat #2603; RRID: AB_490795
Anti-phospho-mTOR(Ser2481) antibody used at 1:1000	Cell Signaling Technology	Cat # 2974; RRID: AB_2262884
Anti-phospho-mTOR(Ser2448)Antibody used at 1:1000	Cell Signaling Technology	Cat # 2971; RRID: AB_330970
Anti-mTOR antibody used at 1:1000	Cell Signaling Technology	Cat # 2972; RRID: AB_330978
Anti-ACADM antibody used at 1:500	Abcam	Cat # ab118183
Anti-ACADVL antibody used at 1:500	Abcam	Cat # ab118183
Anti-HADHA antibody used at 1:500	Abcam	Cat # ab118183
Anti-PPAR-a antibody used at 1:1000	Abcam	Cat # ab191226
Anti-PPAR-a antibody used at 1:1000	Abcam	Cat # ab24509; RRID: AB_448110
Anti-PPAR-γ antibody used at 1:1000	Cell Signaling Technology	Cat # 2435, RRID: AB_2166051
Anti-GAPDH antibody used at 1:2000	Cell Signaling Technology	Cat # 5174; RRID: AB_10622025
Anti-ACTIN antibody used at 1:2000	Cell Signaling Technology	Cat # 4970; RRID: AB_2223172
Rabbit anti-mouse IgG mAb at 1:3000	Cell Signaling Technology	Cat # 58,802; RRID: AB_2799549
Mouse anti-rabbit IgG mAb at 1:3000	Cell Signaling Technology	Cat # 45,262; RRID: AB_2799281
Purified rat anti mouse CD16/CD32	BD Bioscience	Cat # 560051
Anti-mouse CD45 alexa fluor 700 use at 1:100	Biolegend	Cat # 103128; RRID: AB_493715
Anti-mouse F4/80 APC/Cyanine7 use at 1:100	Biolegend	Cat # 123117; RRID: AB_893489
Anti-mouse/human CD11b FITC use at 1:100	BD Bioscience	Cat # 553310; RRID: AB_394774
Anti-mouse CD4 APC/Cyanine7 use at 1:100	Biolegend	Cat # 1004141; RRID: AB_312699
Anti-mouse CD8b APC/Cyanine7 use at 1:100	Biolegend	Cat # 126619; RRID: AB_2563950
Anti-mouse Ly-6G APC use at 1:100	Biolegend	Cat # 127614; RRID: AB_2227348
Anti-human CD45 PE use at 1:100	Biolegend	Cat # 304008; RRID: AB_314396
Anti-human CD14 FITC use at 1:100	Biolegend	Cat # 301804; RRID: AB_31,418
Anti-human CD14 APC/Cyanine7 use at 1:100	Biolegend	Cat # 325620; RRID: AB_830693
Anti-human CD16 PE/Cy5 use at 1:100	Biolegend	Cat # 302009; RRID: AB_314209
DAPI	Boster	Cat # AR1176
BODIPY™ 493/503	Thermo Fisher	Cat #D3922
Donkey anti-mouse IgG (H + L) secondary antibody alexa Fluor® 488 conjugate	Thermo Fisher	Cat # A-21202; RRID: AB_141607
Donkey anti-mouse IgG (H + L) secondary antibody alexa Fluor® 555 conjugate	Thermo Fisher	Cat # A-31570; RRID: AB_2536180
Biological samples
Healthy blood (n = 102)	The First affiliated Hospital of ZhengZhou University	http://fcc.zzu.edu.cn/
Hypertriglyceridemia patient derived blood (n = 8)	The First affiliated Hospital of ZhengZhou University	http://fcc.zzu.edu.cn/
Mouse blood samples (C57/6J background)	Animal center of ZhengZhou University	http://www5.zzu.edu.cn/dwsyzx/info/1012/1027.htm
Critical commercial assays
Fatty acid oxidation assay kit	Abcam	Cat # ab118183
Human IL-6 ELISA MAX™ deluxe	Biolegend	Cat # 430504
Human TNF-α ELISA MAX™ deluxe	Biolegend	Cat # 430204
Mouse IL-6 ELISA MAX™ deluxe	Biolegend	Cat # 431304
Mouse TNF-α ELISA MAX™ deluxe	Biolegend	Cat # 431904
Oligonucleotides
sgRNAs target sequence Ppara1, CACCGCCCGTGATCCCCGCCAGCA, AAACTGCTGGCGGGGATCACGGGC	This paper	https://www.sangon.com/
sgRNAs target sequence Ppara2, CACCGCCGTGCTGGCGGGGATCAC; AAACGTGATCCCCGCCAGCACGGC	This paper	https://www.sangon.com/
sgRNAs target sequence Ppara3, CACCGTAGACACCCTCTCTCCAGCTTC; AAACGAAGCTGGAGAGAGGGTGTCTAC	This paper	https://www.sangon.com/
sgRNAs target sequence Ppara4, CACCGCCTCTTCCATCTGTAGACACCC; AAACGGGTGTCTACAGATGGAAGAGGC	This paper	https://www.sangon.com/
TNFA forward: CTCTTCTGCCTGCTGCA CTTTG	This paper	N/A
TNFA reverse: ATGGGCTACAGGCTTG TCACTC	This paper	N/A
CXCL1 forward: GCCAGTGCTTGCAGACCCT	This paper	N/A
CXCL1 reverse: GGCTATGACTTCGGT TTGGG	This paper	N/A
CCL2 forward: TCATAGCAGCCACCTT CATTC	This paper	N/A
CCL2 reverse: TAGCGCAGATTCTTGGGTTG	This paper	N/A
IL6 forward: ACTCACCTCTTCAGAAC GAATTG	This paper	N/A
IL6 reverse: CCATCTTTGGAAGGTTC AGGTTG	This paper	N/A
IL8 forward: GAGAGTGATTGAGAGTGG ACCAC	This paper	N/A
IL8 reverse: CACAACCCTCTGCACCCAGTTT	This paper	N/A
IL10 forward: GGTTGCCAAGCCTTGTCTGA	This paper	N/A
IL10 reverse: AGGGAGTTCACATGCGCCT	This paper	N/A
GAPDH forward: GCTCCCTCTTTCTTTG CAGC	This paper	N/A
GAPDH reverse: ACCATGAGTCCTTCC ACGAT	This paper	N/A
Tnfa forward: AAAGACCAGGTGGAGTGG AAGAAC	This paper	N/A
Tnfa reverse: CTCAGTGCCGATGGAGTC CGAGTA	This paper	N/A
Cxcl1 forward: CAAGGCTGGTCCATGCTCC	This paper	N/A
Cxcl1 reverse: TGCTATCACTTCCTTTCT GTTGC	This paper	N/A
Ccl2 forward: TTTTGTCACCAAGCTCA AGAGA	This paper	N/A
Ccl2 reverse: ATTAAGGCATCACAGTCC GAGT	This paper	N/A
Il6 forward: TGGGGCTCTTCAAAAGCTCC	This paper	N/A
Il6 reverse: AGGAACTATCACCGGATC TTCAA	This paper	N/A
Il8 forward: ATGACTTCCAAGCTGGCC GTGGCT	This paper	N/A
Il8 reverse: TCTCAGCCCTCTTCAAAA ACTTCTC	This paper	N/A
Il10 forward: CCCATTCCTCGTCACGATCTC	This paper	N/A
Il10 reverse: TCAGACTGGTTTGGGAT AGGTTT	This paper	N/A
Gapdh forward: TCTCTGCTCCTCCCTGTTCC	This paper	N/A
Gapdh reverse: TACGGCCAAATCCGT TCACA	This paper	N/A
Ppara forward: TGGTGTTCGCAGCTGTTTTG	This paper	N/A
Ppara reverse: AGATACGCCCAAATGCACCA	This paper	N/A
pEX5 (pGCMV/EGFP/MCS/Neo) plasmid	GENECAM	N/A
PPARa over-expression plasmid	GENECAM	N/A
Recombinant DNA
Software and algorithms
Seahorse Explorer analaysis program	-	https://www.agilent.com/zh-cn/products/cell-analysis/seahorse-analyzers
FlowJo v.10	FlowJo	https://www.flowjo.com/solutions/flowjo
Imaris v.9	OXFORD	https://imaris.oxinst.com/versions/9
GraphPad prism version 7 for Windows	GraphPad software	https://www.graphpad.com/
ImageJ	-	https://imagej.nih.gov/ij/