TABLE 1.
Strains used in this study.
| Strain | Genotype | Construction1 | References |
| ES114 | Wild type | N/A | Boettcher and Ruby, 1990 |
| JB19 | ES114 litR:erm | N/A | Bose et al., 2007 |
| KB2B1 | Wild type | N/A | Wollenberg and Ruby, 2009 |
| KV8961 | KB2B1 luxQ-E74K (GAA to AAA) | Motile suppressor | This study |
| KV8962 | KB2B1 VF_1200-fs Δ1 bp (341/2,076 nt) | Motile suppressor | This study |
| KV8963 | KB2B1 crp-G185C | Motile suppressor | This study |
| KV8964 | KB2B1 luxO-V106G (GTT to GGT) | Motile suppressor | This study |
| KV8965 | KB2B1 VF_1200-fs Δ1 bp (1678/2,076 nt) | Motile suppressor | This study |
| KV8973 | KB2B1 ΔVF_1200:FRT-Cm | TT KB2B1 with gES114 ΔVF_1200:FRT-Cm2 | This study |
| KV9019 | KB2B1 luxO:pAIA3 | TT KB2B1 with gKV2191 (Hussa et al., 2007) | This study |
| KV9020 | KB2B1 luxQ:Tn5 | TT KB2B1 with gKV4432 (Ray and Visick, 2012) | This study |
| KV9023 | KB2B1 litR:erm | TT KB2B1 with gJB19 (Bose et al., 2007) | This study |
| KV9067 | KB2B1 ΔVF_1200:FRT-Cm litR:erm | TT KV8973 with gJB19 (Bose et al., 2007) | This study |
| KV9105 | KB2B1 Δqrr1:FRT-Erm | TT KB2B1 with gKV8867 (Tepavčević et al., In preparation) | This study |
| KV9115 | KB2B1 ΔVF_1200:FRT-Cm Δqrr1:FRT-Erm | TT KV8973 with gKV8867 (Tepavčević et al., In preparation) | This study |
1TT, TfoX-mediated transformation as described in the section “Materials and Methods”. 2This strain, KV8932, was made using PCR SOEing as described in Visick et al. (2018) with primer sets (templates) as follows: 2577 and 2578 (ES114), 2089 and 2090 (pKV495), and 2579 and 2580 (ES114).