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. 2021 Jun 28;12:690459. doi: 10.3389/fmicb.2021.690459

TABLE 3.

Primers used in this study.

Name Sequence Purpose
945 CGCATACGTTTCTACCGTTTC Overexpress VF_0087
946 CCAAGCTGAACGAAGTGGAC Overexpress VF_0087
2089 CCATACTTAGTGCGGCCGCCTA Amplify antibiotic resistance cassette
2090 CCATGGCCTTCTAGGCCTATCC Amplify antibiotic resistance cassette
2577 TAAAGCAGCAGCTGAGCAAG Amplify sequences upstream of VF_1200
2578 TAGGCGGCCGCACTAAGTATGGTC GTACTTGTTCGAGCAGTAAC Amplify sequences upstream of VF_1200
2579 GGATAGGCCTAGAAGGCCATGGT TAGCGCATATCAAAGAAACCATG Amplify sequences downstream of VF_1200
2580 GTTTGGTGAGTACCAATCGC Amplify sequences downstream of VF_1200
443 CGGTAATACTCCATAAGTTCTTTCAC Confirm ΔsypQ:FRT-Cm
1189 TATTCATCTAGAGTCAGATACC Confirm ΔsypQ:FRT-Cm
2577 TAAAGCAGCAGCTGAGCAAG Confirm ΔVF_1200:FRT-Cm
2580 GTTTGGTGAGTACCAATCGC Confirm ΔVF_1200:FRT-Cm
2425 CCTATTGCAGGGAGCGTGCCAAC Confirm luxO:pAIA3
71 TTATCTTTCATTCCAAGACTGTAG Confirm luxO:pAIA3
908 GCACTGAGAAGCCCTTAGAGCC Confirm luxQ:Tn5
1304 Gatattgctttaggtgctattgatg Confirm luxQ:Tn5
2531 Ggtaccgattaaggaagagctgttaac Confirm litR:Erm
2532 Ggtaccgctgcggaagtatttgaagg Confirm litR:Erm