TABLE 1.

Composition of solutions used for mitochondrial isolation and measuring respiration.

Component	Homogenization buffer	Respiration medium
Sucrose, mmol l–1	250	110
HEPES, mmol l–1	10	20
EGTA, mmol l–1	1	0.5
MgCl2, mmol l–1		1.4
KH2PO4, mmol l–1		10
K-MES, mmol l–1		60
Taurine, mmol l–1		20
BSA (fatty-acid-free)	1%	1%
pH	7.4	7.1
Preparation temperature (°C)	4	Room

All chemicals were purchased from Sigma-Aldrich (St. Louis, MO, United States). pH was adjusted with KOH (5 N).