Skip to main content
. 2021 Jun 28;15:668286. doi: 10.3389/fncel.2021.668286

FIGURE 4.

FIGURE 4

AM251 preserves the number of immature neurons and cell proliferation in the irradiated hippocampus of mice. DCX: Representative images showing density of immature neurons (DCX + cells, brown staining) in the SGZ-GCL of the hippocampus (pink, nuclear staining) at 6 (A1–A4) and 19 weeks (B1–B4) following irradiation. At 6- and 19-weeks postirradiation, significant radiation-induced reductions in the number DCX + immature neurons were found, an effect that was ameliorated significantly by short- (A5) and long-term (B5) AM251 treatments, respectively. BrdU: Representative confocal images showing BrdU + cells (darkly staining) in the SGZ-GCL of the hippocampus at 6 (C1–C4) and 15 weeks (D1–D4) following irradiation. At 6 and 19 weeks postirradiation, significant radiation-induced reductions in the number of BrdU + cells were found which was prevented by short- (C5) and long-term (D5) AM251 treatments, respectively. Data presented as means ± SEM, N = 6. P-values derived from two-way ANOVA followed by Bonferroni correction for multiple comparisons. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.