Skip to main content
. 2021 Jul 6;46(2):180. doi: 10.3892/or.2021.8131

Figure 5.

Figure 5.

RORβ inhibits stemness properties of GCSCs. GC cells were transfected with control, RORβ overexpression vector or RORβ shRNA vector. (A) Images of colospheres of GC cells. Self-renewal capacity of GCSCs was determined using a sphere formation assay. (B and C) mRNA expression levels of CSC markers in RORβ-overexpressing or RORβ-silenced AGS cells and MKN45 cells were analyzed using RT-qPCR and confirmed by western blotting. (D and E) Tumorigenicity of RORβ-overexpressing cells. Nude mice were inoculated with 1×105, 5×105 and 1×106 RORβ-overexpressing AGS cells (n=5). The numbers and volumes of the tumors were observed within 4 weeks. (F) RNA and proteins from the tumors were extracted. Expression levels of EMT markers in the aforementioned tumors were analyzed using RT-qPCR and western blotting. (G) RNA and proteins from the tumors were extracted. Expression levels of GCSC markers in the aforementioned tumors were analyzed using RT-qPCR and western blotting. Data were analyzed using a unpaired Student's t-test. *P<0.05 and **P<0.01. RORβ, retinoic acid-related orphan receptor β; GCSCs, GC stem cells; GC, gastric cancer; CSCs, cancer stem cells; RT-qPCR, reverse transcription-quantitative PCR; ov, overexpression; sh-, short hairpin.