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. 2021 Jun 28;15:676891. doi: 10.3389/fncir.2021.676891

Figure 4.

Figure 4

Reduced NLGN3 expression at inhibitory interneurons to PC synapses in NLGN3-R451C mutant mice. (A–F) Post-embedding immunogold electron microscopy for NLGN3 in wild-type (A–C) and NLGN3-R451C mutant (D–F) mice. (A,D) Indicate PC spines (asterisks) contacting to PF presynaptic terminals labeled for VGluT1. (B,E) Indicate PC spines (asterisks) contacting to CF presynaptic terminals labeled for VGluT2. (C,F) Indicate PC dendrites (PCD) contacting to presynaptic terminals of inhibitory interneurons (InT). Scale bars, 200 nm and 50 nm for inset. (G) Summary bar graphs showing the average number of NLGN3 immunogold particles (n) per 100 μm of the postsynaptic membrane of PF-PC, CF-PC, and InT-PC synapses in wild-type (open circles and columns, N = 3 mice aged 30–35 days) and NLGN3-R451C mutant (orange circles and columns, N = 3 mice aged 30–35 days) mice. Data are expressed as mean ± SD. p = 0.33 for PF-PC, p = 0.61 for CF-PC and *p = 0.035 for InT-PC synapses by Mann-Whitney U test.