. 2021 Jun 29;64(13):9141–9151. doi: 10.1021/acs.jmedchem.1c00362

Table 3. Michaelis–Menten Parameters Determined for the Cephalosporin Conjugates as Substrates of IMP-28, VIM-2, and NDM-1.^a^,^b.

enzyme	substrate	K_M (μM)	k_cat (s^–1)	kcat/K_M (μM^–1·s^–1)	relative kcat/K_M
NDM-1	8	14.0 ± 2	13.1	0.936	100
	9	21.2 ± 4	17.4	0.821	88
VIM-2	8	8.28 ± 2	4.06	0.490	52
	9	4.30 ± 1	2.35	0.546	58
IMP-28	8	129 ± 13	0.386	0.003	0.30
	9	249 ± 7	2.83	0.011	1.2
	10	175 ± 19	41.0	0.234	44
	11	20.5 ± 4	10.8	0.529	56
	12	83.3 ± 16	6.12	0.073	14
	13	219 ± 23	37.0	0.169	18
	15	392 ± 73	23.3	0.059	6.3

Experimental procedure has been described in detail in the Experimental Section.

See the Supporting Information for the Michaelis–Menten graphs.

Table 3. Michaelis–Menten Parameters Determined for the Cephalosporin Conjugates as Substrates of IMP-28, VIM-2, and NDM-1.a,b.