Fig. 1.

Ezetimibe treatments inhibit T. gondii, N. caninum and B. besnoiti tachyzoite proliferation in primary endothelial cells. BUVEC were treated with ezetimibe (2.5, 5, 10 and 20 μm) 48 h before (A) T. gondii, (B) N. caninum or (C) B. besnoiti infection (MOI 1:5). 48 h after infection, the number of tachyzoites present in cell culture supernatants were counted (A–C). Exemplary illustration of T. gondii (A1−A2) N. caninum (B1−B2) or B. besnoiti (C1−C2) meront development at 24 h post infection. Scale bar represents 5 μm. Bars represent means of five biological replicates ± standard deviation. * P ⩽ 0.05; ** P ⩽ 0.01; *** P ⩽ 0.001; **** P ⩽ 0.0001.