Fig. 6.

The induction of NKG2DLs by treatment with MMP inhibitors and limited induction by co-treatment with TGF-β in lung cancer cells. Surface expression levels of NKG2DLs were analyzed using flow cytometry in (A, D) NCI-H23, (B, E) SW-900 and (C, F) A549 lung cancer cells. Lung cancer cells were treated with MMP inhibitors and TGF-β (10 ng/ml) for 18 h. The relative expression ratios were calculated from mean fluorescence intensities (MFI) of treated cells compared to those of control cells. Filled gray, dotted gray, gray and black lines represent isotype, media control, MMP inhibitors 10 μM treatment and TGF-β 10 ng/ml with MMP inhibitors 10 μM treatment, respectively. The experiments were performed three times. (p < 0.05, *; p < 0.01, **)