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. 2021 Jul 12;40:227. doi: 10.1186/s13046-021-02029-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The expression of circ_0084615 in CRC cells and the features of circ_0084615. (A) The expression of circ_0084615 in FHC, SW620, DLD1, SW480 and HT-29 cells was detected by qRT-PCR assay. (B) Circ_0084615 was formed by gene ASPH. (C) The existence of CIRC_0084615 was detected by qRT-PCR assay with divergent or convergent primers. (D and E) The existence of circ_0084615 in the reverse transcription products using random hexamer primers and oligo (dT)18 primers. (F and G) The levels of circ_0084615 and ASPH in SW620 and DLD-1 cells treated with Act D were examined with qRT-PCR assay. (H and I) The levels of circ_0084615 and ASPH in SW620 and DLD-1 cells treated with or without RNase R were detected by qRT-PCR assay. (J and K) The expression of circ_0084615 in the cytoplasm and nucleus of SW620 and DLD-1 cells was analyzed with subcellular fraction analysis. **P < 0.01, ***P < 0.001