| Component | Amount (μl) |
|---|---|
| Nuclease-free water | 18 |
| 4× Ultralow input ligation buffer | |
| (Qiagen Ultralow Input Library Kit, 180492) | 25 |
| QIAseq Adapter (1:10 diluted) | |
| (Qiagen, 180985) | 2 |
| Ultralow Input Ligase | |
| (Qiagen Ultralow Input Library Kit, 180492) | 5 |
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