| Initial denaturation | 98 °C | 2 min |
|---|---|---|
| Denaturation | 98 °C | 20 s |
| Annealing | 60 °C | 30 s |
| Extension | 72 °C | 1 min |
| 12–14 cycles (depends on samples) | ||
| Final extension | 72 °C | 1 min |
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