| Component | Amount (μl) |
|---|---|
| dCTP (10 mM) | 1.5 |
| dGTP (10 mM) | 1.5 |
| dTTP (10 mM) | 1.5 |
| Biotin-14-dATP (0.4 mM, Thermo Fisher Scientific) | 37.5 |
| 5 U μl−1 DNA polymerase I, Large (Klenow) Fragment (NEB) | 8 |
| Total | 50 |
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