| Component | Amount (μl) |
|---|---|
| 10× NEB T4 DNA ligase buffer | 500 |
| 10 mg ml−1 BSA (Thermo Fisher Scientific) | 12 |
| 400 U μl−1 T4 DNA ligase (NEB) | 20 |
| H2O | 6,200 |
| Total | 6,732 |
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