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. 2021 Jul 12;12:81. doi: 10.1186/s40104-021-00606-x

Table 2.

Oligonucleotide primers used in jejunal qRT-PCR analysisa

Gene name Sequence (forward, reverse) Reference
GAPDH 5′-GAGGGTAGTGAAGGCTGCTG-3’ [14]
5′-CCACAACACGGTTGCTGTAT-3’
CLDN1 5′-TGGAGGATGACCAGGTGAAGA-3’ [15]
5′-CGAGCCACTCTGTTGCCATA-3’
MUC2 5′-TTCATGATGCCTGCTCTTGTG-3’ [15]
5′-CCTGAGCCTTGGTACATTCTTGT-3’
IL-6 5′-GACGAGGAGAAATGCCTGACG-3’ [16]
5′-CCGAGTCTGGGATGACCACTTC-3’
IL-10 5′-TCTACACAGATGAGGTCCTGCC-3’ [16]
5′-AGGTGAAGAAGCGGTGACAG-3’

aAbbreviations: CLDN1 claudin-1, GAPDH glyceraldehyde-3-phosphate dehydrogenase, IL-6 interleukin-6, IL-10 interleukin-10, MUC2 mucin 2, OCLD occludin, ZO-1 zonula occludens-1