Fig. 4.

The effects of Zn-loaded bioactive ceramics in promoting the M2 polarization of M$\phi$s. A Immunofluorescence staining images of M$\phi$s seeded on scaffolds for 3 days. CD206 (green) and iNOS (red), DAPI (blue). Scale bar = 40 μm. B The levels of the cytokines IL-1β, TNF-α, and IL-10 in the culture medium of M$\phi$s seeded on scaffolds for 3 days were analyzed by ELISA. PLLA, PLLA scaffold; TCP, TCP/PLLA scaffold; 5%Zn, 5%Zn-loaded TCP/PLLA scaffold; 10%Zn, 10%Zn-loaded TCP/PLLA scaffold; M$\phi$ macrophages; nsno significance; *p < 0.05; **p < 0.01