Table 2.

Annealing positions of the forward and reverse primers of primer pairs 1 and 2

Plasmodium species	GenBank accession number	Annealing positions of the forward and reverse primers of primer pair 1a	Annealing positions of the forward and reverse primers of primer pair 2a
P. falciparum	LR605957.1	Forward: 339–357 Reverse: 434–454	Not applicable
P. vivax	KY923424.1	Forward: 1493–1511 Reverse: 1588–1608	Forward: 1941–1971 Reverse: 2122–2152
P. malariae	AB489194.1	Forward: 1560–1578 Reverse: 1655–1675	Not applicable
P. ovale wallikeri	HQ712053.1	Forward: 1038–1056 Reverse: 1133–1153	Not applicable
P. ovale curtisi	HQ712052.1	Forward: 1038–1056 Reverse: 1133–1153	Not applicable
P. knowlesi	LR701176.1	Forward: 340–358 Reverse: 435–455	Forward: 781–811 Reverse: 962–992
P. cynomolgi	AB444125.1	Forward: 1605–1623 Reverse: 1700–1720	Forward: 2051–2081 Reverse: 2232–2262

See main text for the primer sequences. The expected amplicon sizes of primer pairs 1 and 2 are 116 and 212 base pairs, respectively

^aAccording to the deposited sequences in GenBank under the shown accession numbers