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. 2021 Jul 9;30:09636897211027819. doi: 10.1177/09636897211027819

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — MSLN mediated the effects of Metformin on inhibiting the IL-6/STAT3 signaling. Western blot results showed the expression of MSLN, IL-6, p-STAT3 and STAT3 in untreated and 5 mM metformin-treated group. Quantitative analysis of the effects of metformin on IL-6 and p-STAT3 expression in control and 5 mM metformin-treated SKOV3 cells, data were showed in mean ± SEM, N = 5, ****P < .0001. All data were normalized to GAPDH and fold change was expressed in relation to the untreated group. Western blot results showed the expression of MSLN, IL-6, p-STAT3 and STAT3 in control (NC) and MSLN knock-out (KO) cells treated with 0 or 5 mM metformin. Quantitative analysis of the effects of metformin on IL-6 and p-STAT3 expression in control (NC) and MSLN knock-out (KO) SKOV3 cells treated with 0 or 5 mM metformin, data were showed in mean ± SEM, N = 5, ****P < .0001. All data were normalized to GAPDH and fold change was expressed in relation to the untreated group. Western blot results showed the expression of STAT3 in STAT3 deletion SKOV3 cells with or without metformin administration. SKOV3 cell viability was determined by CCK-8 at the indicated time point.