TABLE 3.
In silico analysis of Leishmanial genes selected from qPCRa
| S. No. | Protein | % identity (Mus musculus) | % identity (Homo sapiens) | Contiguous stretch of >11 amino acids |
|---|---|---|---|---|
| 1. | DNA polymerase ε subunit B | 26 | 25 | No |
| 2. | Pyruvate Carboxylase | 29 | 30 | No |
| 3. | p‐nitrophenylphosphatase | 36 | 35 | No |
| 4. | Aldehyde dehydrogenase | 46 | 48 | Yes |
| 5. | Coproporphyrinogen III oxidase | 45 | 46 | Yes |
| 6. | Fatty acid desaturase | 44 | 46 | Yes |
| 7. | gp63 Leishmanolysin | 27 | 24 | No |
| 8. | Methionine aminopeptidase | 60 | 57 | Yes |
| 9. | NAD+synthase | 26 | 22 | No |
| 10. | Thiol‐dependent reductase‐1 | 24 | 33 | No |
| 11. | 2‐Oxoisovalerate dehydrogenase | 46 | 46 | Yes |
| 12. | Adenine phosphoribosyltransferase | 47 | 42 | Yes |
| 13. | Adenylate kinase | 37 | 46 | No |
| 14. | ARP 2/3 kinase | 43 | 49 | No |
| 15. | Iron superoxide dismutase | 38 | 41 | Yes |
| 16. | Phosphatidic acid phosphatase | 40 | 36 | No |
| 17. | Ubiquitin‐conjugating enzyme E2 | 71 | 70 | Yes |
| 18. | HGPRT | None | None | No |
| 19. | LmjF_36_4610 | 39 | 42 | No |
| 20. | LmjF.36.3850 | 34 | 44 | No |
| 21. | LmjF_33_2620 | 33 | 32 | No |
Protein sequences of listed Leishmanial genes were subjected to blastp with mouse and human proteome for sequence alignment and similar stretch of amino acids.