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. 2021 May 3;13(4):1398–1407. doi: 10.1111/os.13000

Fig. 4.

Fig. 4

PSTPIP2 overexpression inhibits ERK phosphorylation and restores inflammatory cytokine levels in HG/IL‐1β‐treated rat synovial fibroblasts. (A) Rat synovial fibroblasts were infected by lentivirus vectors and treated by NG (5.5 mM) or HG (25 mM) and IL‐1β (5 ng/mL). Western blotting analysis of PSTPIP2 protein in synovial fibroblasts for all groups. (B) ERK inhibitor U0126 was added to the treated synovial fibroblasts. Western blotting analysis of p‐ERK, ERK and β‐actin proteins in synovial fibroblasts for all groups. (C–E) ELISA analysis of TNF‐α (C), IL‐6 (D) and IL‐1β (E) levels in synovial fibroblasts for all groups. **P < 0.01, ***P < 0.001, ****P < 0.0001. n = 3. NG, normal glucose; HG, high glucose; LV‐NC, negative control lentivirus transfected; LV‐PSTPIP2, PSTPIP2 overexpression lentivirus transfected.