FIGURE 1.

High levels of CCR8 expression discriminate Foxp3⁺ Treg cells within subcutaneously implanted syngeneic MC38 colorectal adenocarcinoma tumours. (a) Representative flow cytometry (left) and replicate measurements (right) of CCR8 expression on indicated CD4⁺ and CD8⁺ Tcell subsets within tumours and spleens of MC38 tumour‐bearing animals at day 21 following tumour implantation. (b) Representative flow cytometry (left) and replicate measurements (right) of CCR8 antibody staining on Treg and CD4⁺ Tconv and CD8⁺T cells within MC38 tumours of WT and Ccr8 ^−/− animals at day 21 following tumour implantation. Data are representative of 2 independently repeated experiments. Bars and error represent mean and SEM. Student's t test; ***P < 0·001; ****P < 0·0001; ns, not significant