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. Author manuscript; available in PMC: 2021 Jul 12.
Published in final edited form as: Mol Cell. 2021 Jan 19;81(5):1100–1115.e5. doi: 10.1016/j.molcel.2020.12.033

Figure 3. Detailed interactions of LshCas13a with crRNA:anti-tag RNA duplex.

Figure 3.

(A) Surface views of the interfaces between Cas13a and duplex formed by crRNA and anti-tag RNA.

(B) Recognition of tag:anti-tag duplex by the Linker and Helical-II domains.

(C and D) Interactions between Cas13a and base pairs 2–6 (C) and 7–15 (D) in the guide:target duplex.

(E) Surface views of pre-target-bound LshCas13a-crRNA complex, with interactions between crRNA tag region and LshCas13a shown in the zoomed-in panel.

See also Figure S3.