Figure 1.

Expression of HyPer7 in yeast.A, expression of roGFP2, roGFP2-Tsa2ΔC_R, and HyPer7 under the TEF promoter in the WT BY4742 strain. Immunoblotting with anti-GFP and anti-Pgk1 antibodies. B, fluorescence (520 nm) emitted by WT BY4742 yeast cells expressing either roGFP2-Tsa2ΔC_R (upper panels) or HyPer7 (lower panels), after excitation at 405 (left panels) and 488 nm (middle panels). The scale bar represents 4 μm. C, fluorescence excitation spectra (emission: 520 nm) of yeast cells expressing roGFP2-Tsa2ΔC_R (left panel) or HyPer7 (right panel). Cells were left untreated (black line) or treated with 10 mM H₂O₂ (red line). Dashed vertical lines indicate the position of the 405- and 488-nm laser lines. Data in this figure are representative of n = 3 independent experiments with n = 3 independent replicates each. ev, empty vector.