Figure 3.

pH sensitivity of HyPer7 in yeast.A, response of BY4742 cells expressing HyPer7 (left panel) or SypHer7 (right panel) to exogenously applied H₂O₂ boli in the concentration range of 10 to 1000 μM. B, response of BY4742 cells expressing HyPer7 (left panel) or SypHer7 (right panel) to exogenously applied pH buffers in the range between 4 and 9. C, fluorescence excitation spectra (emission: 520 nm) of yeast cells expressing HyPer7 or SypHer7, collected at pH 6. Cells were left untreated or treated with 10 mM H₂O₂. Data in this figure are representative of n = 3 independent experiments with n = 3 independent replicates each.