TABLE 3.
Concentration-response of propofol on GABAAR activation and deactivation.
Data shown represent the average ±S.E.M.
| GABAAR | Patch Leak | Response Peak Amplitude | Response Steady-State Amplitude | Desensitization Extent (SS/Peak) | Deactivation τ | N |
|---|---|---|---|---|---|---|
| pA | pA | pA | % | ms | ||
| Rapid application of 1 mM GABA for 0.5 sec: propofol concentration set 1 | ||||||
| GABA control | 20.9 ± 7.3 | 133 ± 36 | 51.1 ± 12.3 | 39.3 ± 2.0 | 129 ± 11 | 8 |
| 0.5 μM PRO | 28.9 ± 13.5 | 100 ± 20 | 40.4 ± 8.9 | 39.6 ± 1.3 | 116 ± 13 | 8 |
| 2 μM PRO | 16.7 ± 4.5 | 109 ± 32 | 45.6 ± 14.9 | 40.0 ± 1.7 | 128 ± 13 | 6 |
| 10 μM PRO | 18.8 ± 4.8 | 96.0 ± 25.2 | 42.1 ± 11.6 | 43.1 ± 2.2 | 208 ± 20 | 6 |
| 50 μM PRO | 16.0 ± 3.0 | 63.9 ± 17.8 | 23.5 ± 7.4 | 33.8 ± 2.7 | 331 ± 21 | 7 |
| PRO washout | 15.9 ± 2.8 | 66.7 ± 22.6 | 29.2 ± 11.3 | 41.8 ± 1.7 | 143 ± 25 | 6 |
| Rapid application of 1 mM GABA for 0.5 sec: propofol concentration set 2 | ||||||
| GABA control | 30.6 ± 6.2 | 93.6 ± 6.8 | 30.5 ± 8.1 | 31.8 ± 7.7 | 130 ± 27 | 6 |
| 1 μM PRO | 36.5 ± 6.4 | 72.2 ± 18.0 | 24.2 ± 6.6 | 34.9 ± 6.7 | 110 ± 15 | 6 |
| 5 μM PRO | 34.3 ± 8.2 | 37.8 ± 9.1 | 11.1 ± 2.4 | 30.0 ± 5.1 | 198 ± 29 | 5 |
| 20 μM PRO | 42.1 ± 8.0 | 21.5 ± 4.6 | 6.1 ± 2.2 | 25.2 ± 6.6 | 328 ± 53 | 5 |
| 100 μM PRO | 29.0 ± 5.9 | 8.9 ± 1.2 | 5.8 ± 7.5 | — | 415 ± 226 | 5 |
| PRO washout | 24.8 ± 10.1 | 20.1 ± 6.3 | 5.3 ± 3.4 | 25.2 ± 13.5 | 129 ± 12 | 3 |