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. Author manuscript; available in PMC: 2022 May 15.
Published in final edited form as: J Immunol. 2021 May 3;206(10):2271–2276. doi: 10.4049/jimmunol.2100141

Figure 2. Forced expression of a rearranged TCRβ chain in RAG2-KO CD4+CD8+ DP cells results in cell expansion.

Figure 2.

(A) Schematic of the experimental approach. (B) Cell counts of RAG2-KO DPs (clones 1 and 4) retrovirally-transduced with empty vector (dTomato), TCR alpha chain (TRA 1383i), or TCR beta chain (TRB 1383i) per 10,000 input of CD34+ cells. Transduced T-lineage cells (dTomato+) were first sorted for CD7+CD5+CD4+CD8+ double positive cells and then re-cultured on OP9-DL4–7FS for 10 days. (n=3 of three independent experiments).