TABLE 2:

Photobleaching effect on DRAQ5 and Acridine Orange

Time point	Median (IQR) fluorescence values
	Acridine Orange	DRAQ5
baseline	100% (100–100%)	100% (100–100%)
1 min	77% (73–89%)	99% (98–99%)
2 min	70% (66–80%)	98% (96–99%
3 min	66% (60–74%)	98% (95–99%)
4 min	63% (54–69%)	98% (94–98%)
5 min	59% (51–65%)	97% (92–97%)
6 min	58% (48–62%)	97% (91–97%)
7 min	56% (44–59%)	96% (90–96%)
8 min	54% (43–56%)	95% (89–96%)
9 min	53% (41–54%)	94% (88–95%)
10 min	51% (40–52%)	93% (87–94%)

Fluorescence intensity values in mean percentages over time. Photobleaching was induced by consistent illumination for 10min for DRAQ5 stained tissue (638 nm), and AO stained tissue (488 nm).

IQR: Interquartile range