Table 3.
Statistical analysis of the mean BNMN and MNf of mCRC patients at the beginning (before), the middle (inter) and at the end (after) of the treatment for all mCRC patients and according to their disease response (progressive disease, stable disease, partial response, and complete response).
| mCRC | Sampling Point | BN cells scored | Mean ± SE | G | P | |
|---|---|---|---|---|---|---|
| All patients | BNMN | Before | 55,000 | 29.3 ± 9.07 | ||
| Inter | 55,000 | 19.4 ± 6.86 | 3.90 | 0.04 | ||
| After | 55,000 | 19.6 ± 8.46 | 3.73 | 0.05 | ||
| MN cells | Before | 55,000 | 32.3 ± 9.97 | |||
| Inter | 55,000 | 22.2 ± 8.28 | 3.65 | 0.05 | ||
| After | 55,000 | 22.6 ± 9.72 | 3.35 | 0.06 | ||
|
CBPI ± SE
(mean ± SE) |
Before | 1.32 ± 0.004 | ||||
| Inter | 1.32 ± 0.004 | |||||
| After | 1.30 ± 0.002 | |||||
| Progressive disease | BNMN | Before | 13,000 | 28.8 ± 10.03 | ||
| Inter | 13,000 | 21.3 ± 6.3 | 2.20 | 0.14 | ||
| After | 13,000 | 20.9 ± 9.66 | 2.46 | 0.11 | ||
| MN cells | Before | 13,000 | 31.3 ± 10.76 | |||
| Inter | 13,000 | 24.6 ± 10.93 | 1.60 | 0.20 | ||
| After | 13,000 | 23.6 ± 10.92 | 2.13 | 0.14 | ||
|
CBPI ± SE
(mean ± SE) |
Before | 1.29 ± 0.001 | ||||
| Inter | 1.32 ± 0.004 | |||||
| After | 1.31 ± 0.003 | |||||
| Stable disease | BNMN | Before | 18,000 | 30,2 ± 8,38 | ||
| Inter | 18,000 | 20,3 ± 6,4 | 3.77 | 0.05 | ||
| After | 18,000 | 20.1 ± 8.77 | 3.93 | 0.04 | ||
| MN cells | Before | 18,000 | 33.1 ± 9.35 | |||
| Inter | 18,000 | 23.1 ± 7.39 | 3.48 | 0.06 | ||
| After | 18,000 | 23 ± 10.02 | 3.55 | 0.06 | ||
|
CBPI ± SE
(mean ± SE) |
Before | 1.32 ± 0.003 | ||||
| Inter | 1.31 ± 0.002 | |||||
| After | 1.30 ± 0.001 | |||||
| Partial Response | BNMN | Before | 23,000 | 29.0 ± 9.54 | ||
| Inter | 23,000 | 17.8 ± 5.98 | 5.15 | 0.02 | ||
| After | 23,000 | 18.7 ± 7.88 | 4.29 | 0.03 | ||
| MN cells | Before | 23,000 | 32,5 ± 10.51 | |||
| Inter | 23,000 | 20.6 ± 7.4 | 5.16 | 0.02 | ||
| After | 23,000 | 22.0 ± 9.30 | 3.94 | 0.04 | ||
|
CBPI ± SE
(mean ± SE) |
Before | 1.33 ± 0.006 | ||||
| Inter | 1.31 ± 0.005 | |||||
| After | 1.30 ± 0.001 |
Values in bold font indicate statistically significant differences (P < 0.05) compared with the controls or as indicated. G indicates 2POi ln(Oi/Ei), where ‘Oi’ is the observed frequency in a cell, ‘Ei’ is the expected frequency under the null hypothesis, ‘ln’ denotes the natural logarithm and the sum is taken over all non-empty cells. SE, standard error; BN, binucleated cells (for each patient 1,000 BN cells were scored); BNMN, binucleated cells with micronuclei.