REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Ultra-LEAF™ Purified anti-human TNF-α Antibody	Biolegend	Biolegend Cat#502815 Clone MAb1 RRID:AB_2814397 (BioLegend Cat. No. 502805)
Ultra-LEAF™ Purified anti-human IFN-γ Antibody	Biolegend	Biolegend Cat#506532 Clone B27 RRID:AB_2801092 (BioLegend Cat. No. 506532)
Ultra-LEAF™ Purified anti-human IL-6 Antibody	Biolegend	Biolegend Cat# 501126 Clone MQ2-1385 RRID:AB_2810626 (BioLegend Cat. No. 501126)
IL-27 Goat anti-Human, Polyclonal, R&D Systems™	RnD systems	RnD systems Cat# Af2526 Goat anti-human polyclonal antibody
InVivoMAb mouse IgG1 isotype control	BioXCell	BioXCell Cat#BE0083 Clone MOPC-21 RRID: AB_1107784
InVivoMAb anti-human PD-1 (CD279)	BioXCell	BioXCell Cat#BE0188 Clone J116 RRID AB_10950318
InVivoMAb anti-human PD-L1 (B7-H1)	BioXCell	BioXCell Cat#BE0285 Clone 29E.2A3 RRID AB_2687808
InVivoMAb mouse IgG2b isotype control	BioXCell	BioXCell Cat#BE0086 Clone MPC-11 RRID AB_1107791
InVivoMAb anti-human/monkey CD28	BioXCell	BioXCell Cat#BE0291 Clone CD28.2 RRID AB_2687814
InVivoMAb anti-human CD3	BioXCell	BioXCell Cat#BE0291Clone OKT-3 RRID AB_1107632
APC-Cy™7 Mouse Anti-Human CD45	BD	BD Biosciences Cat# 557833, RRID:AB_396891Clone 2D1
Alexa Fluor® 700 anti-human CD3 antibody, BioLegend	Biolegend	BioLegend Cat# 300424, RRID:AB_493741 Clone UCHT1
Brilliant Violet 421™ anti-human CD8 antibody, BioLegend	Biolegend	BioLegend Cat# 344748, RRID:AB_2629584 Clone SK1
Brilliant Violet 711™ anti-human CD4 antibody, BioLegend	Biolegend	BioLegend Cat# 317440, RRID:AB_2562912 Clone OKT4
APC anti-human CD103 (Integrin alphaE) antibody	Biolegend	BioLegend Cat# 350216, RRID:AB_2563907 Clone BER-ACT8
Brilliant Violet 605™ anti-human CD39 antibody	Biolegend	BioLegend Cat# 328236, RRID:AB_2750430 Clone A1
PE anti-human CD279 (PD-1) antibody	Biolegend	BioLegend Cat# 329905, RRID:AB_940481 Clone EH12.2H7
PE/Dazzle™ 594 anti-human CD366 (Tim-3) antibody	Biolegend	BioLegend Cat# 345034, RRID:AB_2565886 Clone F38-2E2
BV650 anti-CD223 LAG3	Biolegend	BioLegend Cat# 369315, RRID:AB_2632950 Clone 11C3C65
FITC Anti-human CD49b	Biolegend	BioLegend Cat# 359306, RRID:AB_2562531 Clone P1E6-C5
Brilliant Violet 605™ anti-human CD25 antibody	Biolegend	BioLegend Cat# 302631, RRID:AB_11123913 Clone BC96
PerCP/Cyanine5.5 anti-human CD127 (IL-7Ralpha) antibody	Biolegend	BioLegend Cat# 351321, RRID:AB_10900253 Clone A019D5
Mouse Anti-Human Epcam/trop-1 Monoclonal antibody, Fluorescein Conjugated	RnD systems	R and D Systems Cat# FAB9601 F, RRID:AB_2246506 Clone 158206
FITC anti-human CD31 antibody	Biolegend	BioLegend Cat# 303104, RRID:AB_314330 Clone WM59
Human Fibroblast Activation Protein alpha /FAP PE-conjugated Antibody	RnD systems	RnD systems Cat# FAB3715P Clone # 427819
CD90 Antibody, anti-human, VioBlue®, REAfinity™	Miltenyi	Miltenyi Biotec Cat# 130–114-866, RRID:AB_2726816 Clone [REA897]
FITC anti-human HLA-A,B,C antibody	Biolegend	BioLegend Cat# 311404, RRID:AB_314873 Clone W6/32
Brilliant Violet 650™ anti-human HLA-DR antibody	Biolegend	BioLegend Cat# 307649, RRID:AB_2562544 Clone L243
CD273 (B7-DC) Monoclonal Antibody (MIH18), PE,	Invitrogen	Thermo Fisher Scientific Cat# 12–5888-42, RRID:AB_10853342 Clone MIH18
CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), APC, eBioscience™	eBioscience	Thermo Fisher Scientific Cat# 17–5983-42, RRID:AB_10597586 Clone MIH1
PerCP/Cyanine5.5 anti-human CD73 (Ecto-5’-nucleotidase) antibody	Biolegend	BioLegend Cat# 344013, RRID:AB_2561756 Clone AD2
PE anti-human IFN-gamma antibody	Biolegend	BioLegend Cat# 502509, RRID:AB_315234 Clone 4S.B3
PerCP/Cyanine5.5 anti-human IL-10 antibody	Biolegend	BioLegend Cat# 501417, RRID:AB_2561285 Clone JES3-9D7
Alexa Fluor(R) 488 anti-human TNF-alpha antibody	Biolegend	BioLegend Cat# 502915, RRID:AB_493121 clone 2A5
Pacific Blue™ anti-human/mouse Granzyme B antibody	Biolegend	BioLegend Cat# 515408, RRID:AB_2562196 Clone GB11
BV605 Mouse IgG1, κ Isotype Control antibody	BD	BD Biosciences Cat# 562652, RRID:AB_2714005 Clone X40
Ki-67 Monoclonal Antibody (20Raj1), eBioscience™	eBioscience	Thermo Fisher Scientific Cat# 14–5699-82, RRID:AB_2016711 Clone 20Raj1
PE anti-human FOXP3 antibody	Biolegend	BioLegend Cat# 320108, RRID:AB_492986 Clone 206D
Brilliant Violet 711™ anti-human IL-2 antibody	Biolegend	BioLegend Cat# 500345, RRID:AB_2616638 Clone MQ1-17H12
PerCP/Cyanine5.5 Rat IgG1, κ Isotype Ctrl Antibody	Biolegend	Biolegend Cat# 400426 Clone RTK 7021
Alexa Fluor® 700 anti-human IL-2 antibody	Biolegend	BioLegend Cat# 500320, RRID:AB_528929 Clone MQ1-17H12
Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC) Antibody	Biolegend	Biolegend Cat# 400129 Clone MOPC-21
APC Rat IgG2a, κ Isotype Ctrl Antibody	Biolegend	BioLegend Cat# 400512, RRID:AB_2814702 Clone [RTK2758]
PE Mouse IgG1, κ Isotype Ctrl Antibody	Biolegend	BioLegend Cat# 400112, RRID:AB_2847829Clone MOPC-21
Human TruStain FcX™ (Fc Receptor Blocking Solution) antibody	Biolegend	BioLegend Cat# 422302, RRID:AB_2818986
Brilliant Violet 605™ anti-human CD326 (Ep-CAM) antibody	Biolegend	BioLegend Cat# 324224, RRID:AB_2562518 Clone 9C4
APC/Cyanine7 anti-human IL-17A antibody	Biolegend	BioLegend Cat# 512319, RRID:AB_10612577 Clone BL168
Fixation Buffer	Biolegend	Biolegend Cat # 420801
Zombie UV Viability Kit	Biolegend	Biolegend Cat # 423108
ELISA MAX deluxe Human IFN-gamma ELISA	Biolegend	Biolegend Cat # 430104
ELISA MAX deluxe Human IL-10 ELISA	Biolegend	Biolegend Cat # 430604
ELISA MAX deluxe Human IL-6 ELISA	Biolegend	Biolegend Cat # 430504
Cyto-last buffer	Biolegend	Biolegend Cat # 422501
One comp eBeads	Thermo Fisher	Thermor Fisher Cat # 01–1111-42
Human IL-27 DuoSet ELISA	RnD systems	RnD systems Cat # DY2526-05
Cytofix/cytoperm Fixation/Permeabilization Solution Kit	BD	BD Cat # 554714
HBSS	Gibco	Gibco Cat # 14170–088
PBS – cal – mg	Gibco	Gibco Cat # 14190–094
RPMI	Gibco	Gibco Cat # 31870–025
DMEM	Gibco	Gibco Cat # 21969–035
CD4 microbeads human	Miltenyi	Miltenyi Cat # 130–045-101
CD8 microbeads human	Miltenyi	MiltenyiCat # 130–045-201
IL-2 recombinant protein	Gibco	Gibco Cat # PHC0026
FCS	Gibco	Gibco Cat # 10500–064
Pen-Strep	Gibco	Gibco Cat # 15140–122
L-glutamine	Gibco	Gibco Cat # 25030–024
eBioscience™ Cell Stimulation Cocktail (500X)	Thermo Fisher	Thermo Fisher Cat # 00–4970-93
Collagenase IV	Worthington	Merck Cat # C5138
Collagenase I	Sigma	Merck Cat # SCR 103
DNAse	Sigma	Merck Cat # DN25
Hyaluronidase	Stem Cell technologies	Stem cell technologies Cat # 7461
Lymphoprep	Stem Cell technologies	Stem cell Catalog # 07801
RBC lysis buffer	Sigma	Merck Cat# 11814389001
Falcon™ Cell Strainers 100 uM	Thermo Fisher	Falcon Cat # 10282631
Falcon™ Cell Strainers 70 uM	Thermo Fisher	Falcon Cat # 10788201