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. 2021 Jul 8;12(1):1884–1899. doi: 10.1080/21505594.2021.1949883

Figure 4.

Figure 4.

Multistep phosphorylation of His-Asp in fungi. After the HPK detects the stimulus signal, ATP is used as the donor to phosphorylate a conserved his residue. Subsequently, the phosphate group is transferred to the Asp residue of the same HPK receptor domain and then transferred to the Asp residue of the RR receptor domain through the his residue of intermediate transfer protein. Four phosphorylation events occur in sequence, forming the four-step phosphate transfer (His-Asp-His-Asp)